Alessandra Scagliarini · 
| Address: |
Department of Veterinary Public Health and Animal Pathology, University of Bologna Via Tolara di sopra 50 40064 Ozzano Emilia (Bo) |
|
Tel: Fax: |
+39 051 2097083 +39 051 2097039 |
Skills and competences
Biotechnological Laboratory
- genetic amplification using PCR and RT-PCR and Real time PCR
- genomic analysis with restriction enzymes and sequence analysis
- protein pattern analysis
- study on the molecular bases of antiviral resistence mechanisms
Virology Laboratory and Cell culture laboratory
- wide collection of animal cell cultures and lines
- production of primary cell cultures starting from different animal tissues
- production of skin organotypic cultures
- qualified high-titre virus stocks (7-8 log TCID50/ml)
- reference strains and field strains available for the most part of the viral species
- antiviral assays using synthetic and natural compounds
- cytotoxicity testing
- testing for valid stop and storage conditions
- isolation and characterization of the viral agents
- interference assays
- TCID50 assays testing serial dilutions
- extended culture times to prevent false negative results and for ultra sensitive detection of viruses
- Real Time PCR protocols for the absolute quantification od viral nucleic acid
