Laura Calzà · 
| Address: |
DIMORFIPA, University of Bologna Via Tolara di Sopra, 50 40064 Ozzano Emilia (BO) |
|
Tel: Fax: |
+39 051 2097947 +39 051 2097953 |
Skills and competences
Regenerative medicine for the nervous system
- Repair through endogenous stem cells:
- new molecules and drug combinations
- environmental strategies
- Repair through exogenous stem cells:
- mesenchymal stem cells, scaffolds and secretory properties
Stem cells as diagnostic tool (2D and 3D culturing; apoptosis, proliferation, differentiation)
- Animal embryonic stem cells
- Human and animal neural stem cells
- Human and animal mesenchymal stem cells
In vitro, ex vivo systems for drug screening (apoptosis, survival, gene expression regulation, phenotype, mitochondial function, etc)
- Primary cultures of glial and neural cells
- Cell lines
- Cocultures and "artificial" skin (Dr. Scagliarini, DSPVSA)
Available techniques
- Pharmacological, molecular and genetic manipulations on cell cultures (antisense oligonucleotide, transfection)
- Molecular biology tecnhiques:
- DNA and RNA analysis: extraction, RT-PCR, real-time PCR
- protein analysis: extraction, western blotting, ELISA, multi-array (Mesoscale system)
- transfection vector preparation and enzymatic assay
Image analysis and quantification
- Morphometry and and microdensitometry
- Confocal laser scan microscopy, including time-lapse imaging and quantification
